mEos3.2-N1
(Plasmid #54525)

• Purpose: (Empty Backbone) Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580
• Depositing Labs: Michael Davidson, Tao Xu
• Publication: Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.

Backbone

• Vector backbone: mEos3.2-N1
• Backbone size (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • mEos3.2 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: CMV
• Tag / Fusion Protein:
  • mEos3.2 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEos3.2-N1 Data Supplement
• A portion of this plasmid was derived from a plasmid made by: T. Xu; PMID 22581370
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

mEos3.2 = mEos2 + I102N/H158E/Y189A. Excitation = 507 / 572; Emission = 516 / 580