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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 485, Emission: 507
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonesfGFP-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- sfGFP (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- sfGFP (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byG.S. Waldo; PMID 16369541
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 485; Emission = 507
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sfGFP-pBAD was a gift from Michael Davidson & Geoffrey Waldo (Addgene plasmid # 54519 ; http://n2t.net/addgene:54519 ; RRID:Addgene_54519) -
For your References section:
Engineering and characterization of a superfolder green fluorescent protein. Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Nat Biotechnol. 2006 Jan;24(1):79-88. doi: 10.1038/nbt1172 10.1038/nbt1172 PubMed 16369541
