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Addgene

Sirius-N1
(Plasmid #54506)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54506 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Sirius-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • Sirius (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • Sirius (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 355; Emission = 424

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sirius-N1 was a gift from Michael Davidson (Addgene plasmid # 54506 ; http://n2t.net/addgene:54506 ; RRID:Addgene_54506)

  • For your References section:

    An ultramarine fluorescent protein with increased photostability and pH insensitivity. Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T. Nat Methods. 2009 May;6(5):351-3. doi: 10.1038/nmeth.1317. Epub 2009 Apr 6. 10.1038/nmeth.1317 PubMed 19349978
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