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Addgene

mAmetrine-N1
(Plasmid #54505)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54505 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    mAmetrine-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • mAmetrine (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mAmetrine (C terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PMID 18425137; PIs M.W. Davidson and R.E. Campbell
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 406; Emission = 526

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mAmetrine-N1 was a gift from Robert Campbell & Michael Davidson (Addgene plasmid # 54505 ; http://n2t.net/addgene:54505 ; RRID:Addgene_54505)
  • For your References section:

    Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Nat Methods. 2008 May . 5(5):401-3. 10.1038/nmeth.1207 PubMed 18425137