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Addgene

mOrange-N1 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 54499 with EcoRI-HF and NotI-HF.

Our digests display the correct banding pattern:

EcoRI-HF single digest: 1 band at 4746

NotI-HF single digest: 1 band at 4746

EcoRI-HF / NotI-HF double digest: 2 bands - 762 and ~4kb