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pFA6a-SRM (sortase recognition motif)-kanMX6
(Plasmid #54489)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 2513
  • Vector type
    yeast genome targeting
  • Selectable markers
    Neomycin (select with G418) ; Geneticin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SRM (sortase-recognition motif)
  • Species
    Synthetic
  • Insert Size (bp)
    33
  • Tag / Fusion Protein
    • kanMX6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (unknown if destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on the plasmids from Mark Hochstrasser for epitope tagging in S. cerevisiae:
http://www.addgene.org/browse/article/2501/

sortase sequence = LPETG
full sortase recognition motif = GSLPETGGGRR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-SRM (sortase recognition motif)-kanMX6 was a gift from Eri Sakata (Addgene plasmid # 54489 ; http://n2t.net/addgene:54489 ; RRID:Addgene_54489)
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