YFP(159-238)-gamma-7 in pcDNAI/Amp
(Plasmid
#54473)
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PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNAI/Amp
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 5233
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Modifications to backboneA BglII site was introduced to into the polylinker of pcDNAI/Amp 3' to the BamHI site.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP(159-238)-gamma-7
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Alt nameGNG7
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SpeciesH. sapiens (human); Aequorea victoria
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Insert Size (bp)433
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MutationGgamma-7 was amplified by PCR, which added a BamHI site and a linker sequence (Arg-Ser) to the 5' end and a 3' BglII site. Cloning into the BglII site of YFP(159-238) destroyed the BamHI site.
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Entrez GeneGNG7
- Promoter CMV
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Tag
/ Fusion Protein
- YFP(159-238) was fused to the amino terminus of Ggamma-7. (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypEYFP was purchased from Clontech. Gamma-7 cDNA was obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville, PA.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP(159-238)-gamma-7 in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 54473 ; http://n2t.net/addgene:54473 ; RRID:Addgene_54473) -
For your References section:
Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH. J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. 10.1074/jbc.M401432200 PubMed 15136579