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Addgene

CyclinB-GFP
(Plasmid #54465)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVT
  • Backbone manufacturer
    Carmen Williams (Addgene plasmid #32374)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CyclinB
  • Alt name
    pOD021
  • Species
    M. musculus (mouse)
  • Mutation
    K67E and I150T mutations compared to GenBank reference sequence NP_114172.1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CyclinB-GFP was a gift from Michael Lampson (Addgene plasmid # 54465 ; http://n2t.net/addgene:54465 ; RRID:Addgene_54465)

  • For your References section:

    Increased CDK1 activity determines the timing of kinetochore-microtubule attachments in meiosis I. Davydenko O, Schultz RM, Lampson MA. J Cell Biol. 2013 Jul 22;202(2):221-9. doi: 10.1083/jcb.201303019. Epub 2013 Jul 15. 10.1083/jcb.201303019 PubMed 23857768
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