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Addgene

LII ins 4-5
(Plasmid #54380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAMP (Genart)
  • Backbone manufacturer
    Geneart
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    insulator sequence, dummy 4-5
  • Species
    Synthetic

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LII/LIV dummy, contain 2 repeats of a palindromic 16 bp sequence. These nucleotides have been described to act as an insulator sequence in plants, thus preventing the influence of neighboring promoters on transcriptional activation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LII ins 4-5 was a gift from Martin Parniske (Addgene plasmid # 54380 ; http://n2t.net/addgene:54380 ; RRID:Addgene_54380)

  • For your References section:

    A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M. PLoS One. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218. eCollection 2014. 10.1371/journal.pone.0088218 PubMed 24551083
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