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Addgene

LIIalpha F 4-5
(Plasmid #54352)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54352 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Xpre-S (pICH50505)
  • Backbone manufacturer
    Icon Genetics
  • Vector type
    plant expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

binary expression vector. LII/LIV backbone. ccdB cassettes (ccdB and chloramphenicol resistance gene) were added between the borders by Esp3I cut-ligation, flanked by appropriate type IIS fusion sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIIalpha F 4-5 was a gift from Martin Parniske (Addgene plasmid # 54352 ; http://n2t.net/addgene:54352 ; RRID:Addgene_54352)

  • For your References section:

    A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M. PLoS One. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218. eCollection 2014. 10.1371/journal.pone.0088218 PubMed 24551083
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