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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Backbone manufacturerParniske lab
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
L1 backbone. For blunt-end subcloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57 (Gent) was a gift from Martin Parniske (Addgene plasmid # 54338 ; http://n2t.net/addgene:54338 ; RRID:Addgene_54338) -
For your References section:
A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants. Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M. PLoS One. 2014 Feb 13;9(2):e88218. doi: 10.1371/journal.pone.0088218. eCollection 2014. 10.1371/journal.pone.0088218 PubMed 24551083