Flag_HsIRE1a_pBabePuro
(Plasmid #54337)

• Purpose: N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector
• Depositing Lab: David Ron
• Publication: Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 10.1073/pnas.1217611110. Epub 2013 Mar 4.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Addgene plasmid # 1764
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IRE1a
• Alt name: IRE1
• Alt name: IRE1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 2949
• Mutation: The endogenous IRE1a signal sequence (aa1-18) was replaced with mouse preprotrypsin signal sequence
• Entrez Gene: ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • preprotrypsin signal sequence (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pLXSN 5
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry