pSUMO-YHRC
(Plasmid
#54336)
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Purpose(Empty Backbone) Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneModified pET24
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Backbone manufacturerNovagen
- Backbone size (bp) 6436
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Modifications to backboneKanR promoter changed to facilitate G418 selection in yeast. CEN/ARS sequence introduced from the pRS-series for stable replication and segregation in yeast. 6His-SUMO (yeast gene: SMT3) introduced in pET24 MCS.
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Vector typeBacterial Expression
- Promoter T7-LacO
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Selectable markersNeomycin (select with G418) ; G418
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Tag
/ Fusion Protein
- 6His-SUMO (yeast gene: SMT3) (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUMO-YHRC was a gift from Claes Andréasson (Addgene plasmid # 54336 ; http://n2t.net/addgene:54336 ; RRID:Addgene_54336) -
For your References section:
A versatile bacterial expression vector designed for single-step cloning of multiple DNA fragments using homologous recombination. Holmberg MA, Gowda NK, Andreasson C. Protein Expr Purif. 2014 Jun;98:38-45. doi: 10.1016/j.pep.2014.03.002. Epub 2014 Mar 12. 10.1016/j.pep.2014.03.002 PubMed 24631626
Map uploaded by the depositor.
