pBS SK mCherryROSAbsr
(Plasmid
#54321)
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PurposeEncodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS SK(+)
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 5613
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Vector typeMammalian Expression, Mouse Targeting, CRISPR, TALEN
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry and BlasticidinS-resistance
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS SK mCherryROSAbsr was a gift from Silvestro Conticello (Addgene plasmid # 54321 ; http://n2t.net/addgene:54321 ; RRID:Addgene_54321) -
For your References section:
An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system. Niccheri F, Pecori R, Conticello SG. Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y. 10.1007/s00018-017-2524-y [pii] PubMed 28421278
Map uploaded by the depositor.
