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mEmerald-Vinculin-N-21
(Plasmid #54304)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54304 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEmerald-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    NM_003373.3
  • Species
    H. sapiens (human)
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 487; Emission = 509. Localization data: Vinculin - Focal Adhesions

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-Vinculin-N-21 was a gift from Michael Davidson (Addgene plasmid # 54304 ; http://n2t.net/addgene:54304 ; RRID:Addgene_54304)

  • For your References section:

    A role for actin arcs in the leading-edge advance of migrating cells. Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B, Lippincott-Schwartz J. Nat Cell Biol. 2011 Apr;13(4):371-81. doi: 10.1038/ncb2205. Epub 2011 Mar 20. 10.1038/ncb2205 PubMed 21423177
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