mEmerald-VEGFR2-N
(Plasmid #54298)

• Purpose: Localization: Membrane Receptor, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Davidson mEmerald (unpublished)

Backbone

• Vector backbone: mEmerald-N1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VEGFR2
• Alt name: Kinase insert domain receptor
• Alt name: NM_002253.2
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note VEGFR2 contains a V297I mutation. This is a common gene variant.

Kinase insert domain receptor . Excitation = 487; Emission = 509. Localization data: Membrane Receptor