mEmerald-PCNA-19-SV40NLS-4
(Plasmid #54223)

• Purpose: Localization: Nucleus, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. Epub 2010 Jun 7.

Backbone

• Vector backbone: mEmerald
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA-19 SV40NLS
• Alt name: Human Proliferating Cell Nuclear Antigen
• Alt name: NM_002592.2
• Species: H. sapiens (human)
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mEmerald (N terminal on backbone)
  • 3xSV40NLS (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEmerald-PCNA-19-SV40NLS-4 Data Supplement

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Human Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat. Excitation = 487; Emission = 509. Localization data: Nucleus