mEmerald-PCNA-19-NLS-4
(Plasmid
#54222)
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PurposeLocalization: Nucleus, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54222 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonemEmerald
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCNA-19 NLS
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Alt nameHuman Proliferating Cell Nuclear Antigen
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Alt nameNM_002592.2
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SpeciesH. sapiens (human)
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Entrez GenePCNA (a.k.a. ATLD2)
- Promoter CMV
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Tags
/ Fusion Proteins
- mEmerald (N terminal on backbone)
- SV40NLS (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Human Proliferating Cell Nuclear Antigen + SV40 NLS. Excitation = 487; Emission = 509. Localization data: Nucleus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-PCNA-19-NLS-4 was a gift from Michael Davidson (Addgene plasmid # 54222 ; http://n2t.net/addgene:54222 ; RRID:Addgene_54222) -
For your References section:
G2 phase chromatin lacks determinants of replication timing. Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM. J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. Epub 2010 Jun 7. 10.1083/jcb.201002002 PubMed 20530209