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Addgene

mEmerald-MyosinIIC-N-18
(Plasmid #54195)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54195 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEmerald-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyosinIIC
  • Alt name
    NM_001271540
  • Species
    M. musculus (mouse)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 487; Emission = 509. Localization data: Cytoskeleton

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-MyosinIIC-N-18 was a gift from Michael Davidson (Addgene plasmid # 54195 ; http://n2t.net/addgene:54195 ; RRID:Addgene_54195)

  • For your References section:

    NMII forms a contractile transcellular sarcomeric network to regulate apical cell junctions and tissue geometry. Ebrahim S, Fujita T, Millis BA, Kozin E, Ma X, Kawamoto S, Baird MA, Davidson M, Yonemura S, Hisa Y, Conti MA, Adelstein RS, Sakaguchi H, Kachar B. Curr Biol. 2013 Apr 22;23(8):731-6. doi: 10.1016/j.cub.2013.03.039. Epub 2013 Apr 4. 10.1016/j.cub.2013.03.039 PubMed 23562268
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