mEmerald-MCM4-N-22
(Plasmid
#54168)
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PurposeLocalization: Nucleus, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEmerald-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCM4
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Alt nameNM_008565.3
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 487; Emission = 509. Localization data: Nucleus
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-MCM4-N-22 was a gift from Michael Davidson (Addgene plasmid # 54168 ; http://n2t.net/addgene:54168 ; RRID:Addgene_54168) -
For your References section:
Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM. J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. 10.1083/jcb.201007111 PubMed 21220507