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Addgene

mEmerald-MCM3-N-22
(Plasmid #54166)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54166 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    mEmerald-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCM3
  • Alt name
    NM_008563.2
  • Species
    M. musculus (mouse)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 487; Emission = 509. Localization data: Nucleus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-MCM3-N-22 was a gift from Michael Davidson (Addgene plasmid # 54166 ; http://n2t.net/addgene:54166 ; RRID:Addgene_54166)
  • For your References section:

    Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM. J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. 10.1083/jcb.201007111 PubMed 21220507