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Addgene

Emerald-MannII-N-10
(Plasmid #54150)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54150 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Emerald-N1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MannII
  • Alt name
    NM_008549.2
  • Species
    M. musculus (mouse)
  • Mutation
    aa 1-112 of MannII aa 1-112 of MannII
  • Entrez Gene
    Man2a1 (a.k.a. Mana-2, Mana2, Map-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Emerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Excitation = 487; Emission = 509. Localization data: Mannosidase/Golgi

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Emerald-MannII-N-10 was a gift from Michael Davidson (Addgene plasmid # 54150 ; http://n2t.net/addgene:54150 ; RRID:Addgene_54150)

  • For your References section:

    Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Planchon TA, Gao L, Milkie DE, Davidson MW, Galbraith JA, Galbraith CG, Betzig E. Nat Methods. 2011 May;8(5):417-23. doi: 10.1038/nmeth.1586. Epub 2011 Mar 4. 10.1038/nmeth.1586 PubMed 21378978
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