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Addgene

mEmerald-RatLC3B-C-7
(Plasmid #54147)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54147 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    mEmerald-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RatLC3B
  • Alt name
    Microtubule-associated proteins 1A and 1B light chain 3
  • Alt name
    U05784.1
  • Species
    R. norvegicus (rat)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Microtubule-associated proteins 1A and 1B light chain 3. Excitation = 487; Emission = 509. Localization data: Microtubule-Binding Protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-RatLC3B-C-7 was a gift from Michael Davidson (Addgene plasmid # 54147 ; http://n2t.net/addgene:54147 ; RRID:Addgene_54147)
  • For your References section:

    Internalized gap junctions are degraded by autophagy. Fong JT, Kells RM, Gumpert AM, Marzillier JY, Davidson MW, Falk MM. Autophagy. 2012 May 1;8(5):794-811. doi: 10.4161/auto.19390. Epub 2012 May 1. 10.4161/auto.19390 PubMed 22635056