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Addgene

mEmerald-Keratin-17
(Plasmid #54134)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54134 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEmerald
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keratin
  • Alt name
    NM_199187.1
  • Species
    H. sapiens (human)
  • Mutation
    V282L
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 487; Emission = 509. Localization data: Cytokeratin (18)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-Keratin-17 was a gift from Michael Davidson (Addgene plasmid # 54134 ; http://n2t.net/addgene:54134 ; RRID:Addgene_54134)

  • For your References section:

    Advances in fluorescent protein technology. Shaner NC, Patterson GH, Davidson MW. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60 10.1242/jcs.005801 PubMed 18057027
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