mEmerald-H3-23
(Plasmid #54115)

• Purpose: Localization: Nucleus/Histones, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Davidson mEmerald (unpublished)

Backbone

• Vector backbone: mEmerald
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H3
• Alt name: Human Histone H3
• Alt name: NM_003537.3
• Species: H. sapiens (human)
• Entrez Gene: H3C2 (a.k.a. H3/l, H3C1, H3C10, H3C11, H3C12, H3C3, H3C4, H3C6, H3C7, H3C8, H3FL, HIST1H3B)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEmerald-H3-23 Data Supplement
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Human Histone H3. Excitation = 487; Emission = 509. Localization data: Nucleus/Histones