mEmerald-Golgi-7
(Plasmid #54108)

• Purpose: Localization: Golgi Complex, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Davidson mEmerald (unpublished)

Backbone

• Vector backbone: mEmerald
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Golgi
• Alt name: GalT Target
• Alt name: NM_001497.3
• Species: H. sapiens (human)
• Mutation: aa 1-82 of NM_001497.3
• GenBank ID:
• Entrez Gene: B4GALT1 (a.k.a. B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEmerald-Golgi-7 Data Supplement
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

GalT Target. Excitation = 487; Emission = 509. Localization data: Golgi Complex