mEmerald-GFAP-C-18
(Plasmid
#54106)
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PurposeLocalization: Intermediate Filaments, Excitation: 487, Emission: 509
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 54106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonemEmerald-C1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFAP
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Alt nameGlial Fibrillary Acidic Protein
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Alt nameNM_002055.2
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SpeciesH. sapiens (human)
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GenBank ID
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Entrez GeneGFAP (a.k.a. ALXDRD)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Glial Fibrillary Acidic Protein . Excitation = 487; Emission = 509. Localization data: Intermediate Filaments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-GFAP-C-18 was a gift from Michael Davidson (Addgene plasmid # 54106 ; http://n2t.net/addgene:54106 ; RRID:Addgene_54106)