mEmerald-ER-3
(Plasmid #54082)

• Purpose: Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Davidson mEmerald (unpublished)

Backbone

• Vector backbone: mEmerald
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ER
• Alt name: NM_004343.3
• Species: H. sapiens (human)
• Mutation: ER Signal Peptide
• Entrez Gene: CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEmerald-ER-3 Data Supplement
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

. Excitation = 487; Emission = 509. Localization data: Endoplasmic Reticulum