mEmerald-EB3-7
(Plasmid
#54075)
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PurposeLocalization: MT End Binding Protein, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEmerald
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEB3
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Alt nameNM_012326.2
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SpeciesH. sapiens (human)
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Entrez GeneMAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 487; Emission = 509. Localization data: MT Binding Protein
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-EB3-7 was a gift from Michael Davidson (Addgene plasmid # 54075 ; http://n2t.net/addgene:54075 ; RRID:Addgene_54075) -
For your References section:
Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 2013 May 26. 10.1038/nmeth.2488 PubMed 23708387