mEmerald-Cx43-7
(Plasmid
#54055)
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PurposeLocalization: Gap Junctions, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54055 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEmerald
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCx43
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Alt nameAlpha-1 Connexin 43
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Alt nameNM_012567.2
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SpeciesR. norvegicus (rat)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Rat Alpha-1 Connexin 43. Excitation = 487; Emission = 509. Localization data: Gap Junctions
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-Cx43-7 was a gift from Michael Davidson (Addgene plasmid # 54055 ; http://n2t.net/addgene:54055 ; RRID:Addgene_54055) -
For your References section:
Internalized gap junctions are degraded by autophagy. Fong JT, Kells RM, Gumpert AM, Marzillier JY, Davidson MW, Falk MM. Autophagy. 2012 May 1;8(5):794-811. doi: 10.4161/auto.19390. Epub 2012 May 1. 10.4161/auto.19390 PubMed 22635056