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Addgene

mEmerald-Cx32-7
(Plasmid #54054)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54054 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    mEmerald
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cx32
  • Alt name
    Beta-1 Connexin 32
  • Alt name
    NM_001097642.2
  • Species
    H. sapiens (human)
  • Entrez Gene
    GJB1 (a.k.a. CMTX, CMTX1, CX32)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human Beta-1 Connexin 32. Excitation = 487; Emission = 509. Localization data: Gap Junctions

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-Cx32-7 was a gift from Michael Davidson (Addgene plasmid # 54054 ; http://n2t.net/addgene:54054 ; RRID:Addgene_54054)

  • For your References section:

    In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors. Fort AG, Murray JW, Dandachi N, Davidson MW, Dermietzel R, Wolkoff AW, Spray DC. J Biol Chem. 2011 Jul 1;286(26):22875-85. doi: 10.1074/jbc.M111.219709. Epub 2011 May 2. 10.1074/jbc.M111.219709 PubMed 21536677
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