mEmerald-CENPB-N-22
(Plasmid
#54037)
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PurposeLocalization: Nucleus/Centromeres, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54037 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEmerald-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCENPB
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Alt nameNM_001810.5
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SpeciesH. sapiens (human)
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Mutationaa 1-169 of CENPB (DNA Binding Domain)
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Entrez GeneCENPB
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 487; Emission = 509. Localization data: Nucleus/Centromeres
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-CENPB-N-22 was a gift from Michael Davidson (Addgene plasmid # 54037 ; http://n2t.net/addgene:54037 ; RRID:Addgene_54037) -
For your References section:
The fluorescent protein palette: tools for cellular imaging. Day RN, Davidson MW. Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 2009 Aug 4. 10.1039/b901966a PubMed 19771335