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PurposeLocalization: Membrane, Excitation: 487, Emission: 509
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonemEmerald-N1
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaveolin
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Alt nameNM_001753
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SpeciesH. sapiens (human)
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Entrez GeneCAV1 (a.k.a. BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21)
- Promoter CMV
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 487; Emission = 509. Localization data: Membrane
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEmerald-Caveolin-N-10 was a gift from Michael Davidson (Addgene plasmid # 54026 ; http://n2t.net/addgene:54026 ; RRID:Addgene_54026) -
For your References section:
Overexpression of caveolin-1 is sufficient to phenocopy the behavior of a disease-associated mutant. Hanson CA, Drake KR, Baird MA, Han B, Kraft LJ, Davidson MW, Kenworthy AK. Traffic. 2013 Jun;14(6):663-77. doi: 10.1111/tra.12066. Epub 2013 Apr 7. 10.1111/tra.12066 PubMed 23469926