mEmerald-Alpha-Actinin-C-14
(Plasmid #53990)

• Purpose: Localization: Non-muscle Alpha Actinin, Excitation: 487, Emission: 509
• Depositing Lab: Michael Davidson
• Publication: Davidson mEmerald (unpublished)

Backbone

• Vector backbone: mEmerald-C1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alpha-Actinin
• Alt name: NM_001102.3
• Species: H. sapiens (human)
• GenBank ID: NM_001102.3
• Entrez Gene: ACTN1 (a.k.a. BDPLT15)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEmerald (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEmerald-Alpha-Actinin-C-14 Data Supplement

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Excitation = 487; Emission = 509. Localization data: Non-muscle Alpha Actinin

Please note that in the attachment provided by the depositing laboratory, the alpha actinin 1 sequence is listed as transcript variant 3; however, the sequence provided and the insert in the plasmid is transcript variant 2 (NM_001102.3).