pCMV3FLAG_hGTPBP4
(Plasmid #53974)

• Purpose: Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.
• Depositing Lab: Martin Dorf
• Publication: Li et al Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.

Backbone

• Vector backbone: pCMV-3Tag-6
• Backbone manufacturer: Agilent (formerly Stratagene)
• Backbone size w/o insert (bp): 5202
• Total vector size (bp): 7107
• Modifications to backbone: Translation initiates from the vector's n-terminal FLAGs; the native ATG of the GTPBP4 CDS is deleted. The native TAG stop is included, followed by GAT then the backbone after the ApaI site.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GTPBP4
• Alt name: GTP binding protein 4
• Alt name: NGB; CRFG; NOG1
• Species: H. sapiens (human)
• Insert Size (bp): 1905
• Entrez Gene: GTPBP4 (a.k.a. RP11-38M7.5, CRFG, NGB, NOG1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3x FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: T3, CMV forward
• 3′ sequencing primer: T7 (41bp from insert), BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry