pCR2.1-INS-904
(Plasmid
#53970)
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Purpose904 bp insert from human INS gene used as a control for methylation-specific PCR assays
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1-TOPO
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Backbone manufacturerLife Technologies
- Total vector size (bp) 4837
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman INS gene partial
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SpeciesH. sapiens (human)
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Insert Size (bp)904
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GenBank IDNM_000207.2 NG_007114.1
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Entrez GeneINS (a.k.a. IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4)
- Promoter lac
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1-INS-904 was a gift from Kevin Ferreri (Addgene plasmid # 53970 ; http://n2t.net/addgene:53970 ; RRID:Addgene_53970) -
For your References section:
Development of a quantitative methylation-specific polymerase chain reaction method for monitoring beta cell death in type 1 diabetes. Husseiny MI, Kuroda A, Kaye AN, Nair I, Kandeel F, Ferreri K. PLoS One. 2012;7(10):e47942. doi: 10.1371/journal.pone.0047942. Epub 2012 Oct 29. 10.1371/journal.pone.0047942 PubMed 23144715
