pCMVhPOP1_3Flag
(Plasmid #53968)

• Purpose: Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.
• Depositing Lab: Martin Dorf
• Publication: Li et al Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.

Backbone

• Vector backbone: pCMV-3Tag-8
• Backbone manufacturer: Agilent (formerly Stratagene)
• Backbone size w/o insert (bp): 5170
• Total vector size (bp): 8249
• Modifications to backbone: XhoI-insert-NotI-ClaI multiple cloning site substituted for commercial SacI-XhoI MCS. CTCGACGCCACC sequence before the POP1 CDS is the SalI into XhoI insertion, with an added Kozak sequence. The stop codon is removed from the POP1 coding sequence before the NotI site leaving 3072bp. 3xFLAGs are in frame with restriction sites and gene CDS.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POP1
• Alt name: processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
• Species: H. sapiens (human)
• Insert Size (bp): 3078
• GenBank ID: BC011529.1
• Entrez Gene: POP1 (a.k.a. ANXD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xhoi (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV, T3 (25bp from gene CDS)
• 3′ sequencing primer: T7, BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Obtained from DF/HCC DNA Resource Core of Harvard Medical School.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MGC clone. GI: 15079365, IMAGE ID: 3853321, GenBank Accession: BC011529.1, Original Clone ID MGC:17365.