Skip to main content
Addgene

pcDNA5-FRT-GFP-mCherry-3pGW
(Plasmid #53965)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53965 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    CmlR
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method TOPO Cloning
  • 5′ sequencing primer ATTCGCCACCATGGTGAGCAAGG
  • 3′ sequencing primer TCACTTGTACAGCTCATCCATGCC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method TOPO Cloning
  • 5′ sequencing primer ATGGTGAGCAAGGGCGAGGAGGATAA
  • 3′ sequencing primer CTTACTTGTACAGCTCGTCCATG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-GFP-mCherry-3pGW was a gift from Saeed Tavazoie (Addgene plasmid # 53965 ; http://n2t.net/addgene:53965 ; RRID:Addgene_53965)

  • For your References section:

    Systematic Identification of Regulatory Elements in Conserved 3' UTRs of Human Transcripts. Oikonomou P, Goodarzi H, Tavazoie S. Cell Rep. 2014 Apr 10;7(1):281-92. doi: 10.1016/j.celrep.2014.03.001. Epub 2014 Mar 20. 10.1016/j.celrep.2014.03.001 PubMed 24656821
Related items:
Commonly requested with: