pJG/BETA MHC promoter
(Plasmid
#53963)
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Purpose(Empty Backbone) transgene expression driven by BETA MHC promoter
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II KS+
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Backbone manufacturerStratagene
- Backbone size (bp) 2961
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Modifications to backboneaddition of beta MHC 5'UTR region, HpaI cloning site, and hgH polyA site.
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Vector typeMammalian Expression
- Promoter beta MHC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJG/BETA MHC promoter was a gift from Jeffrey Robbins (Addgene plasmid # 53963 ; http://n2t.net/addgene:53963 ; RRID:Addgene_53963) -
For your References section:
In vivo analysis of the murine beta-myosin heavy chain gene promoter. Rindt H, Gulick J, Knotts S, Neumann J, Robbins J. J Biol Chem. 1993 Mar 5;268(7):5332-8. PubMed 8444907
Map uploaded by the depositor.
