MDM2p-Mdm2-YFP
(Plasmid #53962)

• Purpose: Expresses YFP tagged Mdm2 from MDM2 promoter
• Depositing Labs: Uri Alon, Galit Lahav
• Publication: Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2004 Jan 18.

Backbone

• Vector backbone: pBSK+
• Backbone size w/o insert (bp): 7661
• Total vector size (bp): 9132
• Modifications to backbone: neomycin resistance gene added
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MDM2
• Species: H. sapiens (human)
• Insert Size (bp): 1485
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
• Promoter: MDM2
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ECO47III (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry