Mtp-p53-ECFP
(Plasmid #53961)

• Purpose: Expresses CFP tagged p53 in mammalian cells
• Depositing Labs: Uri Alon, Galit Lahav
• Publication: Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2004 Jan 18.

Backbone

• Vector backbone: pBSK+
• Backbone size w/o insert (bp): 5914
• Total vector size (bp): 7092
• Modifications to backbone: Hygromycin
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53
• Species: H. sapiens (human)
• Insert Size (bp): 1178
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: metallothionein promoter
• Tag / Fusion Protein:
  • ECFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry