pcDNA4-ATR-C-KD
(Plasmid #53770)

• Purpose: mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant
• Depositing Lab: Aziz Sancar
• Publication: Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.

Backbone

• Vector backbone: pcDNA4.1
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATR
• Alt name: ataxia telangiectasia and Rad3 related
• Species: H. sapiens (human)
• Mutation: C-terminal domain, aa 1398-2644, and kinase dead mutation (K2327R)*
• Entrez Gene: ATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*R1631H polymorphism--not known to affect function