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Purposemammalian expression of flag-tagged ATR C-terminal fragment
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATR
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Alt nameataxia telangiectasia and Rad3 related
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SpeciesH. sapiens (human)
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MutationC-terminal domain, aa 1398-2644*
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Entrez GeneATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
**R1631H polymorphism--not known to affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-ATR-C was a gift from Aziz Sancar (Addgene plasmid # 53769 ; http://n2t.net/addgene:53769 ; RRID:Addgene_53769) -
For your References section:
Preferential binding of ATR protein to UV-damaged DNA. Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8. 10.1073/pnas.102167799 PubMed 12011431