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Addgene

pEGFPN1-Gm129
(Plasmid #53765)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gm129
  • Alt name
    Ciart
  • Species
    M. musculus (mouse)
  • Mutation
    *
  • Entrez Gene
    Ciart (a.k.a. Chrono, Gm129)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that the 2nd amino acid in the insert is missing. This omission is not known to effect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPN1-Gm129 was a gift from Aziz Sancar (Addgene plasmid # 53765 ; http://n2t.net/addgene:53765 ; RRID:Addgene_53765)

  • For your References section:

    Gene model 129 (Gm129) encodes a novel transcriptional repressor that modulates circadian gene expression. Annayev Y, Adar S, Chiou YY, Lieb JD, Sancar A, Ye R. J Biol Chem. 2014 Feb 21;289(8):5013-24. doi: 10.1074/jbc.M113.534651. Epub 2014 Jan 2. 10.1074/jbc.M113.534651 PubMed 24385426
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