slc6a3 probe
(Plasmid #53764)

• Purpose: cRNA probe to detect the slc6a3 transcript
• Depositing Lab: Edward Burton
• Publication: Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51. doi: 10.1016/j.neuroscience.2009.09.014. Epub 2009 Sep 12.

Backbone

• Vector backbone: pBS
• Backbone size w/o insert (bp): 2259
• Total vector size (bp): 3639

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DAT
• gRNA/shRNA sequence: 1380
• Species: D. rerio (zebrafish)

Resource Information

• Supplemental Documents:
  • Gene Bank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert contains S38F mutations P78L, they are not believed to effect the function of the plasmid. The clone has been successfully used
for making a probe that works well for Northern and RNA ISH (Bai 2009,
https://www.ncbi.nlm.nih.gov/pubmed/19755139) but it cannot be used for RNase protection because of the mutations. The clone only contains part of the ORF of DAT and should not be used for over-expression experiments.