pEGFP-C3-Exo84
(Plasmid #53762)

• Purpose: pEGFP-C3 plasmid that expresses Exo84
• Depositing Lab: Channing Der
• Publication: Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi: 10.1016/j.molcel.2013.12.004. Epub 2014 Jan 2.

Backbone

• Vector backbone: pEGFP-C3
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6877
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Exo84
• Alt name: EXOC8
• Alt name: SEC84
• Alt name: Exo84p
• Species: H. sapiens (human)
• Insert Size (bp): 2177
• GenBank ID:
• Entrez Gene: EXOC8 (a.k.a. EXO84, Exo84p, NEDMISB, SEC84)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry