pCS-memb-mCherry
(Plasmid #53750)

• Purpose: Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.
• Depositing Lab: Sean Megason
• Publication: Megason et al Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2x membrane linker
• Promoter: simian CMV IE94
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cut with NotI and transcribe with SP6 for RNA.

CDS of mCherry was PCR amplified to add AgeI at N terminus and SnaBI at C-terminus. Put
this fragment in AgeI/SnaBI cut pCS-memb-cerulean (Addgene plasmid 53749).