pCS-H2B-cerulean
(Plasmid #53748)

• Purpose: Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.
• Depositing Lab: Sean Megason
• Publication: Megason et al Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: histone H2B
• Alt name: histone cluster 2, H2be
• Species: H. sapiens (human)
• Entrez Gene: H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
• Promoter: simian CMV IE94
• Tag / Fusion Protein:
  • Cerulean (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H2B was obtained from Dr. Reinhard Koster.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use NotI/SP6 for sense RNA.

This plasmid was made using a triple ligation of the H2B from XhoI/AgeI of pCS-H2B-EGFP (Addgene plasmid 53744), the cerulean from AgeI/BsrG1 of pCeruleanC1 and pCS from BsrG1/XhoI of pCS-H2B-EGFP. This puts H2B-cerulean in the SnaBI site of pCS.