pCS-H2B-EYFP
(Plasmid
#53746)
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Purposecontains fusion of human histone H2B to EYFP in pCS2+ vector.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
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Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehistone H2B
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Alt namehistone cluster 2, H2be
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SpeciesH. sapiens (human)
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Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter simian CMV IE94
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byH2B was obtained from Dr. Reinhard Koster.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS-H2B-EYFP was a gift from Sean Megason (Addgene plasmid # 53746 ; http://n2t.net/addgene:53746 ; RRID:Addgene_53746) -
For your References section:
In toto imaging of embryogenesis with confocal time-lapse microscopy. Megason SG. Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19. 10.1007/978-1-60327-977-2_19 PubMed 19378112