pEU-Gm23
(Plasmid
#53738)
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PurposeUsed for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 53738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEU-E01
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Backbone manufacturerCell Free Sciences
- Backbone size w/o insert (bp) 3747
- Total vector size (bp) 5525
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert namearginyl-tRNA synthetase
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Alt nameRARS
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SpeciesGlycine max (soybean)
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Insert Size (bp)1824
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GenBank IDFM209045
- Promoter SP6
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Tag
/ Fusion Protein
- His tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEU-Gm23 was a gift from Gabor Igloi (Addgene plasmid # 53738 ; http://n2t.net/addgene:53738 ; RRID:Addgene_53738)